5E9U

Crystal structure of GtfA/B complex bound to UDP and GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	CCD
Collection date	2014-10-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	148.975, 196.005, 215.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.481 - 3.840
R-factor	0.2711
Rwork	0.269
R-free	0.33680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e9t
RMSD bond length	0.005
RMSD bond angle	0.901
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	148.980	148.980	4.050
High resolution limit [Å]	3.840	12.140	3.840
Rmerge	0.385	0.075
Rpim	0.166	0.032	0.876
Total number of observations	448209	13529	66279
Number of reflections	60983
<I/σ(I)>	5.28	23.4
Completeness [%]	100.0	99.5	99.9
Redundancy	7.3	6.4	7.5
CC(1/2)	0.990	0.996	0.581

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	PEG 4000, magnesium chloride, Tris, glycyl-glycyl-glycine