5E9N
Steccherinum murashkinskyi laccase at 0.95 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-31 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.803 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.510, 83.270, 111.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 0.950 |
| R-factor | 0.12209 |
| Rwork | 0.121 |
| R-free | 0.13941 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fpx |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.804 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 1.000 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.070 | 0.668 |
| Number of reflections | 981844 | |
| <I/σ(I)> | 10.44 | 2 |
| Completeness [%] | 94.4 | 96.1 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris pH 8.5, 0.2 ammoniun sulphate, 25% PEG 3350 |
