5E8R

Human HMT1 hnRNP methyltransferase-like protein 6 (S. cerevisiae)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97921
Spacegroup name	P 43
Unit cell lengths	100.244, 100.244, 89.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.550
R-factor	0.2213
Rwork	0.220
R-free	0.25560
RMSD bond length	0.007
RMSD bond angle	1.182
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.550	6.920	2.550
Rmerge	0.171	0.041	0.893
Rmeas	0.198	0.049
Rpim	0.116	0.027	0.606
Total number of observations	91551
Number of reflections	28929
<I/σ(I)>	6.5
Completeness [%]	99.3	95.4	100
Redundancy	3.2	3.2	3.2
CC(1/2)		0.997	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	295	10% PEG 3350, 0.2M MgCl2, 0.1M Sodium Cacadylate, pH6.5