5E7T

Structure of the tripod (BppUct-A-L) from the baseplate of bacteriophage Tuc2009

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 21 3
Unit cell lengths	211.960, 211.960, 211.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.850 - 2.900
R-factor	0.2155
Rwork	0.214
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5E7F RBP domain
RMSD bond length	0.010
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.900	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.187	1.900
Number of reflections	70164
<I/σ(I)>	10.7	1.25
Completeness [%]	99.8	99.3
Redundancy	13.7	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	by mixing 300 nl of protein (Na2HPO4, 10 mM; KH2PO4, 1.8 mM [pH7.4]; NaCl, 137 mM; KCl, 2.7 mM) with 100 nl precipitant solution (2 M Ammonium Sulfate, 0.1 M Na Hepes [pH 7]).