5E7P
Crystal Structure of MSMEG_0858 (Uniprot A0QQS4), a AAA ATPase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2008-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.801, 111.816, 151.705 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.516 - 2.507 |
| R-factor | 0.2082 |
| Rwork | 0.206 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.770 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_833) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.516 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.487 | |
| Number of reflections | 51244 | |
| <I/σ(I)> | 15.9 | 2 |
| Completeness [%] | 97.5 | 95 |
| Redundancy | 4.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | purified protein 6.5 mg/ml was mixed with equal volume of 0.3 M ammonium tartrate dibasic and 25% polyethylene glycol 3350. |
