5E7K
Structure of T. thermophilus 70S ribosome complex with mRNA and cognate tRNALys in the A-site
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.500, 450.100, 621.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 173.719 - 3.200 |
| R-factor | 0.1916 |
| Rwork | 0.191 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wq1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.670 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 200.000 | 173.719 | 3.300 |
| High resolution limit [Å] | 3.200 | 50.000 | 3.200 |
| Rmerge | 0.375 | 0.053 | 4.297 |
| Rmeas | 0.053 | 4.352 | |
| Total number of observations | 103886962 | ||
| Number of reflections | 955810 | 287 | 83729 |
| <I/σ(I)> | 37.7 | 92.31 | 1.1 |
| Completeness [%] | 100.0 | 90.8 | 100 |
| Redundancy | 108.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 20000, PEG 550mme, KSCN, Tris-OAc |
