5E7J

Crystal structure of the active catalytic core of the human DEAD-box protein DDX3 bound to AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115869
Spacegroup name	P 21 21 21
Unit cell lengths	51.350, 89.910, 107.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.007 - 2.230
R-factor	0.2316
Rwork	0.230
R-free	0.26370
RMSD bond length	0.003
RMSD bond angle	0.790
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.007		2.290
High resolution limit [Å]	2.230	9.970	2.230
Rmerge	0.068	0.016	0.483
Rmeas	0.078	0.019	0.596
Total number of observations	96049
Number of reflections	24117	321	1174
<I/σ(I)>	16.99	60.76	2.82
Completeness [%]	96.4	97.6	64.8
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	PEG 3000, sodium citrate