5E7F
Complex between lactococcal phage Tuc2009 RBP head domain and a nanobody (L06)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-07 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.99 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.730, 88.040, 147.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.800 - 2.700 |
R-factor | 0.1948 |
Rwork | 0.194 |
R-free | 0.20790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5efb |
RMSD bond length | 0.008 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.100 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.146 | 1.600 |
Number of reflections | 31023 | |
<I/σ(I)> | 11 | 1.2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | mixing 300 nl of protein (10 mM HEPES, pH 7.5, 150 mM NaCl) with 100 nl precipitant solution (25-30 % PEG4000, 0.2 M Imidazole pH 6.0, or 0.1 M Tris pH 8.0). |