5E7F

Complex between lactococcal phage Tuc2009 RBP head domain and a nanobody (L06)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-07
Detector	PSI PILATUS 6M
Wavelength(s)	0.99
Spacegroup name	P 21 21 21
Unit cell lengths	84.730, 88.040, 147.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.800 - 2.700
R-factor	0.1948
Rwork	0.194
R-free	0.20790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5efb
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.100	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.146	1.600
Number of reflections	31023
<I/σ(I)>	11	1.2
Completeness [%]	99.9	99.9
Redundancy	7.2	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	mixing 300 nl of protein (10 mM HEPES, pH 7.5, 150 mM NaCl) with 100 nl precipitant solution (25-30 % PEG4000, 0.2 M Imidazole pH 6.0, or 0.1 M Tris pH 8.0).