5E70
Crystal structure of Ecoli Branching Enzyme with gamma cyclodextrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.097, 103.221, 186.694 |
| Unit cell angles | 90.00, 91.73, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.330 |
| R-factor | 0.17504 |
| Rwork | 0.170 |
| R-free | 0.21716 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m7x |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.346 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.330 |
| Number of reflections | 126463 |
| <I/σ(I)> | 25 |
| Completeness [%] | 95.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 0.1M HEPES, pH = 7.2 |
