5E6O

Crystal structure of C. elegans LGG-2 bound to an AIM/LIR motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9785
Spacegroup name	P 1 21 1
Unit cell lengths	49.933, 62.351, 67.599
Unit cell angles	90.00, 109.54, 90.00

Refinement procedure

Resolution	47.058 - 1.800
R-factor	0.1806
Rwork	0.177
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.143
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.070	1.830
High resolution limit [Å]	1.800	1.800
Rmerge		0.462
Number of reflections	36467
<I/σ(I)>	18.1	4.1
Completeness [%]	99.0	100
Redundancy	6.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	1.5M sodium citrate tribasic dihydrate