5E61
Structure of amyloid-forming peptide FGAILSS (residues 23-29) from islet amyloid polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2014-04-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 8.770, 9.500, 24.740 |
| Unit cell angles | 88.22, 80.00, 70.34 |
Refinement procedure
| Resolution | 4.380 - 1.790 |
| R-factor | 0.17221 |
| Rwork | 0.167 |
| R-free | 0.21833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.000 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 4.380 | 1.960 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.241 | 0.696 |
| Number of reflections | 647 | |
| <I/σ(I)> | 4.29 | 1.4 |
| Completeness [%] | 93.4 | 72.3 |
| Redundancy | 5.217 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 6.4mg/ml in 20mM Lithium hydroxide and mixed with 0.1M HEPES pH 6.5 and 0.5M Sodium Formate |
