5E5Z

Structure of the amyloid forming peptide LVHSSN (residues

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	291
Detector technology	CCD
Collection date	2010-03-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	9.643, 9.609, 19.029
Unit cell angles	90.00, 101.22, 90.00

Refinement procedure

Resolution	9.459 - 1.664
R-factor	0.1702
Rwork	0.167
R-free	0.19830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.975
Data reduction software	DENZO
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.600
Rmerge	0.076
Number of reflections	1136
<I/σ(I)>	17.86
Completeness [%]	92.9
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20 mg/ml in water and mixed with 0.09 M HEPES pH 7.5, 1.26M tri-sodium citrate, and 10% glycerol