5E5D
Native structure of Xoo1075, a peptide deformylase from Xanthomonas oryzae pv. oryzae
Replaces: 3DLDExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 58.968, 58.968, 266.285 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.400 - 2.600 |
R-factor | 0.18981 |
Rwork | 0.187 |
R-free | 0.24020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sv2 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.913 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.440 | |
Number of reflections | 9011 | |
<I/σ(I)> | 22.5 | 6.6 |
Completeness [%] | 96.9 | 97.4 |
Redundancy | 9.6 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 0.05M Cadmium sulfate, 0.1M HEPES, 2.0M Sodium acetate trihydrate, pH 7.5 |