5E5B

Crystal structure of Human Spt16 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-09-19
Detector	PSI PILATUS 6M
Wavelength(s)	0.979493
Spacegroup name	F 4 3 2
Unit cell lengths	246.570, 246.570, 246.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.642 - 1.840
R-factor	0.1574
Rwork	0.156
R-free	0.17560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3biq
RMSD bond length	0.008
RMSD bond angle	1.216
Data reduction software	xia2
Data scaling software	Aimless (3.3.16)
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.180	87.180	1.880
High resolution limit [Å]	1.840	8.210	1.840
Rmerge	0.082	0.029	0.779
Rpim	0.019	0.007	0.193
Total number of observations	1124024	13455	72859
Number of reflections	56315
<I/σ(I)>	28.1	77.1	4.2
Completeness [%]	100.0	99.1	100
Redundancy	20	17.6	17.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	17% (w/v) PEG 3350, 0.1 M ammonium iodide and 0.1 M sodium acetate pH 5