5E59
Crystal structure of reduced state of a novel disulfide oxidoreductase from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-07 |
| Detector | AGILENT TITAN CCD |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 47.761, 62.922, 86.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.759 - 2.000 |
| R-factor | 0.1596 |
| Rwork | 0.157 |
| R-free | 0.20580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cnw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.903 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.760 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.121 | 0.667 |
| Number of reflections | 18231 | |
| <I/σ(I)> | 19.1 | 6.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 10.1 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 10% PEG 8000 100mM Sodium Actetate 20% Glycerol |
