5E53
Crystal structure of chicken CNTN1 FN1-FN3 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.484, 49.871, 163.302 |
| Unit cell angles | 90.00, 97.12, 90.00 |
Refinement procedure
| Resolution | 43.506 - 2.497 |
| R-factor | 0.2069 |
| Rwork | 0.205 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e7l |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.840 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.497 | 5.810 | 2.497 |
| Rmerge | 0.075 | 0.086 | 0.380 |
| Total number of observations | 171135 | ||
| Number of reflections | 48701 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 99.2 | 99.2 | 92.3 |
| Redundancy | 6.9 | 13.4 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1% (v/v) Tacsimate pH 7.0, 20% (w/v) PEG 3350, 50mM Imidazole pH 6.5 |
