5E4V

Crystal structure of measles N0-P complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 31 2 1
Unit cell lengths	91.140, 91.140, 94.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	78.930 - 2.710
R-factor	0.214
Rwork	0.211
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4co6
RMSD bond length	0.005
RMSD bond angle	0.976
Data reduction software	xia2 (0.3.8.0)
Data scaling software	xia2 (0.3.8.0)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	94.070	94.070	2.860
High resolution limit [Å]	2.710	8.570	2.710
Rmerge	0.123	0.030	0.893
Rpim	0.059	0.015	0.450
Total number of observations	63741	2062	8825
Number of reflections	12618
<I/σ(I)>	7.5	24	1.6
Completeness [%]	100.0	100	100
Redundancy	5.1	4.5	4.9
CC(1/2)	0.996	0.998	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	295	PEG 4000, sodium citrate