5E4P

X-ray Crystal Structure Analysis of Magnetically Oriented Microcrystals of Lysozyme at 1.8 angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	293
Detector technology	CCD
Collection date	2014-06-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	30.110, 49.400, 60.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.165 - 1.792
R-factor	0.1731
Rwork	0.171
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vdq
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.390
High resolution limit [Å]	1.790
Number of reflections	7494
<I/σ(I)>	19.8
Completeness [%]	85.1
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		313	PEG4000, Sodium chloride