5E4I

Crystal structure of mouse CNTN5 Ig1-Ig4 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	179.975, 50.659, 51.015
Unit cell angles	90.00, 101.67, 90.00

Refinement procedure

Resolution	36.963 - 2.600
R-factor	0.2012
Rwork	0.195
R-free	0.26370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jxa
RMSD bond length	0.003
RMSD bond angle	0.830
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.154	0.088	0.435
Total number of observations	90529
Number of reflections	13893
<I/σ(I)>	4.7
Completeness [%]	98.8	99.8	90.7
Redundancy	6.5	7.1	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1% (v/v) Tacsimate pH 7.0, 20% (w/v) PEG 3350, 50 mM Imidazole-HCl pH 6.5