5E3X

Crystal structure of thermostable Carboxypeptidase (FisCP) from Fervidobacterium Islandicum AW-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 2
Unit cell lengths	107.105, 56.757, 89.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.324 - 2.197
R-factor	0.1804
Rwork	0.177
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1WGZ
RMSD bond length	0.007
RMSD bond angle	1.037
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.275
High resolution limit [Å]	2.197	2.197
Rmerge	0.090
Number of reflections	28236
<I/σ(I)>	19.62
Completeness [%]	99.7	98.94
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10%PEG1000 10%PEG3350