5E3P
Crystal structure of DapD from Corynebacterium glutamicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-23 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 91.001, 91.001, 156.815 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 78.810 - 2.010 |
R-factor | 0.1785 |
Rwork | 0.176 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fsx |
RMSD bond length | 0.019 |
RMSD bond angle | 1.894 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 78.810 |
High resolution limit [Å] | 2.010 |
Number of reflections | 24585 |
<I/σ(I)> | 9.68 |
Completeness [%] | 97.6 |
Redundancy | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 200, Li2SO4 |