5E3P

Crystal structure of DapD from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 63 2 2
Unit cell lengths	91.001, 91.001, 156.815
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	78.810 - 2.010
R-factor	0.1785
Rwork	0.176
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fsx
RMSD bond length	0.019
RMSD bond angle	1.894
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall
Low resolution limit [Å]	78.810
High resolution limit [Å]	2.010
Number of reflections	24585
<I/σ(I)>	9.68
Completeness [%]	97.6
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	PEG 200, Li2SO4