5E3E
Crystal structure of CdiA-CT/CdiI complex from Y. kristensenii 33638
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.890, 57.146, 64.302 |
| Unit cell angles | 78.25, 90.21, 79.11 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.17448 |
| Rwork | 0.174 |
| R-free | 0.20222 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.368 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.082 | 0.437 |
| Number of reflections | 71046 | |
| <I/σ(I)> | 8.13 | 2.26 |
| Completeness [%] | 97.1 | 95.5 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | 0.1 M MgCl2, 0.1 M Na citrate pH 5.0, 15% PEG4000, cryo 20% PEG400 |
