5E3D

Crystal structure of the human BRPF1 bromodomain in complex with SEED7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-05
Detector	PSI PILATUS 6M
Wavelength(s)	0.99987
Spacegroup name	P 32 2 1
Unit cell lengths	60.730, 60.730, 62.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.223 - 1.710
R-factor	0.1943
Rwork	0.193
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lc2
RMSD bond length	0.007
RMSD bond angle	0.710
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.220	1.800
High resolution limit [Å]	1.710	1.710
Rmerge	0.031	0.405
Number of reflections	14799
<I/σ(I)>	30.3	5.1
Completeness [%]	99.7	99.1
Redundancy	9.5	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	0.1 M Sodium Acetate, pH5.5, 0.15 M Sodium Nitrate, 20% PEG3350