5E2V
Anti-TAU AT8 FAB with doubly phosphorylated TAU peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-19 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.150, 58.310, 68.560 |
| Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.640 |
| R-factor | 0.16568 |
| Rwork | 0.165 |
| R-free | 0.20793 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e2t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.167 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.680 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.034 | 0.046 |
| Number of reflections | 45789 | |
| <I/σ(I)> | 28.2 | 10 |
| Completeness [%] | 87.2 | 31.1 |
| Redundancy | 3.5 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES, PH 6.5, 25% PEG 4000 |
