5E2U
Structure of anti-TAU AT8 FAB in the presence of phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-03 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 94.690, 107.380, 108.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.18855 |
| Rwork | 0.186 |
| R-free | 0.24829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1ML8 and 3LEY |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.215 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.062 | 0.277 |
| Number of reflections | 21800 | |
| <I/σ(I)> | 19.5 | 5.7 |
| Completeness [%] | 99.4 | 95.7 |
| Redundancy | 6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 20% PEG 3350, 0.2 M AMMONIUM SULFATE, 15% PEG 4000, 0.2 M AMMONIUM SULFATE, 24% PEG 400 |
