5E2T
Crystal structure of anti-TAU antibody AT8 FAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-17 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.870, 108.800, 109.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.20789 |
| Rwork | 0.207 |
| R-free | 0.24647 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e2u |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.306 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.057 | 0.298 |
| Number of reflections | 30818 | |
| <I/σ(I)> | 19.2 | 5.5 |
| Completeness [%] | 94.9 | 77.5 |
| Redundancy | 5.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 20% PEG 3350, 0.2 M CALCIUM CHLORIDE, 20% PEG 3350, 0.2 M CALCIUM CHLORIDE, 25% GLYCEROL |
