5E2J
Crystal structure of single mutant thermostable endoglucanase (D468A) from Alicyclobacillus acidocaldarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Synchrotron site | NSRRC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-28 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.421, 144.374, 158.704 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.100 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ez8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.708 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 73207 | |
| <I/σ(I)> | 26.96 | 2.02 |
| Completeness [%] | 97.0 | 94 |
| Redundancy | 3.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 25 MM MES BUFFER, PH 5, 45% MPD |
