5E21
PDZ2 of LNX2 at 277K,single conformer model
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 277 |
| Detector technology | PIXEL |
| Collection date | 2013-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.912, 39.288, 38.800 |
| Unit cell angles | 90.00, 117.41, 90.00 |
Refinement procedure
| Resolution | 28.950 - 1.011 |
| R-factor | 0.1266 |
| Rwork | 0.126 |
| R-free | 0.13960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vwr |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.879 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.950 | 1.047 |
| High resolution limit [Å] | 1.011 | 1.011 |
| Rmerge | 0.051 | 0.540 |
| Number of reflections | 35251 | |
| <I/σ(I)> | 12.84 | 0.45 |
| Completeness [%] | 77.6 | 3.04 |
| Redundancy | 5.8 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25-29% PEG-300, 57 mM citric acid, 43 mM (Na2HPO4 pH 10.1) |
