5E1T

Crystal structure of TRAF1 TRAF domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	110
Detector technology	CCD
Collection date	2015-02-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	75.352, 79.404, 108.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.523 - 2.800
R-factor	0.2149
Rwork	0.211
R-free	0.26200
RMSD bond length	0.011
RMSD bond angle	1.442
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800	2.800
Number of reflections	15860
<I/σ(I)>	31.2	4.4
Completeness [%]	94.7	91.8
Redundancy	5	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	(NH4)2SO4, NaCl