5E1L
Structural and functional analysis of the E. coli FtsZ interacting protein, ZapC, reveals insight into molecular properties of a novel Z ring stabilizing protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.976, 46.741, 62.910 |
| Unit cell angles | 90.00, 105.74, 90.00 |
Refinement procedure
| Resolution | 37.643 - 2.150 |
| R-factor | 0.2003 |
| Rwork | 0.197 |
| R-free | 0.23120 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.468 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.500 | |
| High resolution limit [Å] | 2.150 | |
| Rmerge | 0.234 | |
| Number of reflections | 9961 | |
| <I/σ(I)> | 11.5 | 4.6 |
| Completeness [%] | 98.0 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.5 M sodium citrate, 0.1 M cacodylate pH 6.5 |
