5E11
Second PDZ domain of Ligand of Numb protein X 2 by Laue crystallography (no electric field)
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 289 |
| Detector technology | CCD |
| Collection date | 2015-03-20 |
| Detector | RAYONIX MX340-HS |
| Wavelength(s) | 1.02-1.16 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.300, 39.450, 39.010 |
| Unit cell angles | 90.00, 117.54, 90.00 |
Refinement procedure
| Resolution | 30.100 - 1.800 |
| R-factor | 0.1325 |
| Rwork | 0.132 |
| R-free | 0.14830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SSRL structure at 277 K (to be deposited) |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.630 |
| Data reduction software | Precognition |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.861 |
| High resolution limit [Å] | 1.800 | 1.797 |
| Rmerge | 0.096 | |
| Number of reflections | 6565 | |
| <I/σ(I)> | 19.6 | 5.9 |
| Completeness [%] | 79.3 | 34.7 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 27-31% PEG 300, 48 mM citric acid, 35 mM NaH2PO4 |
