5E0M
LC8 - Chica (468-476) Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 44.540, 44.540, 205.016 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.573 - 1.650 |
| R-factor | 0.2082 |
| Rwork | 0.207 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bri |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.076 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2006: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.600 | 1.670 |
| High resolution limit [Å] | 1.650 | 1.640 |
| Rmerge | 0.370 | 9.110 |
| Number of reflections | 15900 | |
| <I/σ(I)> | 9.8 | 0.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 33.4 | 24.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2 M lithium sulfate, 0.1 M Bis-Tris, 25% polyethylene glycol 3350 |
