5E0L
LC8 - Chica (415-424) Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 44.695, 44.695, 204.096 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.700 - 1.310 |
| R-factor | 0.1967 |
| Rwork | 0.196 |
| R-free | 0.20150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bri |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.346 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2006: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 1.330 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmerge | 0.081 | 4.450 |
| Number of reflections | 30438 | |
| <I/σ(I)> | 20.6 | 0.4 |
| Completeness [%] | 100.0 | 99.1 |
| Redundancy | 30.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M calcium acetate, 0.1 M sodium cacodylate, 15% polyethylene glycol 8000 |
