5E09
Structural Insight of a Trimodular Halophilic Cellulase with a Family 46 Carbohydrate-Binding Module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979197 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 120.990, 120.990, 204.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.964 - 2.347 |
| R-factor | 0.1817 |
| Rwork | 0.180 |
| R-free | 0.22630 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.550 | 2.430 |
| High resolution limit [Å] | 2.346 | 2.346 |
| Rmerge | 0.050 | 0.401 |
| Number of reflections | 32069 | |
| <I/σ(I)> | 12.13 | 2.02 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 2.0 M ammonium sulfate, 5%(v/v) 2-propanol, 0.1 M Potassium sodium tartrate tetrahydrate |
