5E02

Structure of RNA Helicase FRH a Critical Component of the Neurospora Crassa Circadian Clock

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	76.432, 106.600, 125.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.192 - 3.800
R-factor	0.2819
Rwork	0.278
R-free	0.32060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xgt
RMSD bond length	0.003
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	106.600	106.600	4.010
High resolution limit [Å]	3.800	12.020	3.800
Rmerge	0.149	0.030	1.416
Rpim	0.094	0.020	0.884
Total number of observations	32399	890	4667
Number of reflections	9816
<I/σ(I)>	7.8	29.9	1.2
Completeness [%]	93.7	82.2	94.1
Redundancy	3.3	2.8	3.3
CC(1/2)	0.994	0.999	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	290	sodium citrate, PEG600