5E01
Crystal structure of HiNmlR, a MerR family regulator lacking the sensor domain, bound to palyndromic promoter DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-25 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.953700 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.160, 112.640, 103.790 |
| Unit cell angles | 90.00, 101.84, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.300 |
| R-factor | 0.2202 |
| Rwork | 0.219 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gpv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.860 | 2.160 |
| High resolution limit [Å] | 2.300 | 2.100 |
| Rmerge | 3.264 | |
| Number of reflections | 27932 | |
| Completeness [%] | 77.2 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20 % w/v PEG 3,350; 0.4 M sodium nitrate; 0.1 M Bis-Tris propane pH 7.5; 2 mM TCEP |
