5DZT

Crystal structure of class II lanthipeptide synthetase CylM in complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	80
Detector technology	CCD
Collection date	2014-04-06
Detector	RAYONIX MX-300
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	51.191, 90.699, 246.361
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.200
R-factor	0.24542
Rwork	0.244
R-free	0.27500
RMSD bond length	0.009
RMSD bond angle	1.308
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.200
Number of reflections	51875
<I/σ(I)>	7.9
Completeness [%]	87.4
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M CaCl, 0.1 M HEPES, pH 7.5, 10 mM betaine hydrochloride, and 28% PEG 400; 0.2 M KCl, 0.05 M HEPES pH7.5, 10 mM barium chloride, and 33% 5/4 PO/OH