5DZO

Crystal structure of human T-cell immunoglobulin and mucin domain protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	50.739, 50.739, 84.162
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.004 - 1.301
R-factor	0.1574
Rwork	0.157
R-free	0.17610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.411
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.350
High resolution limit [Å]	1.300	2.800	1.300
Rmerge	0.069	0.068	0.173
Total number of observations	328083
Number of reflections	27042
<I/σ(I)>	10.4
Completeness [%]	97.4	99.5	81.8
Redundancy	12.1	12	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	289	1.5 M sodium nitrate, 0.1 M sodium acetate trihydrate, pH 4.6