5DZB
6beta1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.960, 86.530, 362.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.580 - 3.330 |
| R-factor | 0.2002 |
| Rwork | 0.197 |
| R-free | 0.26480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | predicted structure |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.425 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.580 | 3.420 |
| High resolution limit [Å] | 3.330 | 3.330 |
| Rmerge | 0.110 | 0.666 |
| Number of reflections | 26271 | |
| <I/σ(I)> | 11.61 | 2.88 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 7.08 | 7.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 290 | 20% v/v 1,4 butanediol HEPES pH 7.5, 0.2 M NaCL |
