5DZA
Streptococcus agalactiae AgI/II polypeptide BspA C terminal domain (WT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.920 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 34.030, 205.800, 120.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.140 - 2.190 |
| R-factor | 0.21907 |
| Rwork | 0.218 |
| R-free | 0.23885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Phasing software | MOLREP (5.8.0049) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.140 | 2.260 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.085 | 0.451 |
| Number of reflections | 19496 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 92.3 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.02 M sodium/potassium phosphate and 20% (w/v) PEG 3350 |
