5DYQ
AbyU L73M L139M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2015-05-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9173 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.351, 68.270, 139.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.300 - 1.660 |
| R-factor | 0.19068 |
| Rwork | 0.190 |
| R-free | 0.21136 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.184 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.270 |
| High resolution limit [Å] | 1.660 |
| Rmerge | 0.042 |
| Number of reflections | 65871 |
| <I/σ(I)> | 13.2 |
| Completeness [%] | 86.3 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.02 M magnesium chloride, 0.1 M HEPES 18% w/v poly(acrylic acid) sodium salt MW 5100 |
