5DYM
Crystal structure of a PadR family transcription regulator from hypervirulent Clostridium difficile R20291 - CdPadR_0991 to 1.89 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.420, 92.420, 40.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.369 - 1.894 |
| R-factor | 0.2146 |
| Rwork | 0.213 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xma |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.931 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.370 | 1.962 |
| High resolution limit [Å] | 1.894 | 1.894 |
| Rmerge | 0.018 | 0.382 |
| Number of reflections | 14302 | |
| <I/σ(I)> | 20.27 | 2.15 |
| Completeness [%] | 99.0 | 95.07 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3.1 M NaCl, 100 mM HEPES (pH 7.5) |
