5DYE
CRYSTAL STRUCTURE OF THE FULL LENGTH S156E MUTANT OF HUMAN AQUAPORIN 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 3 1 2 |
| Unit cell lengths | 174.286, 174.286, 100.858 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 150.760 - 3.500 |
| R-factor | 0.28036 |
| Rwork | 0.279 |
| R-free | 0.31096 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.084 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.000 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.095 | 0.799 |
| Number of reflections | 22191 | |
| <I/σ(I)> | 13 | 1.9 |
| Completeness [%] | 98.9 | 99.8 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 281 | 21% PEG 400, 0.1M Tris pH 7.9, 0.1 M NaCl, 3% 1,6 hexanediol and 4% 1,3-propanediol as additive to the drop only |
