5DY1

Crystal of Selenomethionine substituted AmtR from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.981
Spacegroup name	H 3
Unit cell lengths	160.464, 160.464, 52.435
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.110 - 2.651
R-factor	0.1826
Rwork	0.180
R-free	0.22080
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.096
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.232	49.059	2.790
High resolution limit [Å]	2.651	8.380	2.650
Rmerge		0.023	0.339
Rmeas	0.116
Rpim	0.061	0.023	0.301
Total number of observations	47544	1615	4134
Number of reflections	14116
<I/σ(I)>	10.4	37.6	2.1
Completeness [%]	96.4	97.6	82.8
Redundancy	3.4	3.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	0.1 M Na/K phosphate pH 7, 0.2 M NaCl, 6% PEG 8000