5DXZ

Crystal of AmtR from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 32 2 1
Unit cell lengths	69.310, 69.310, 92.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.690 - 2.250
R-factor	0.2209
Rwork	0.220
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dy1
RMSD bond length	0.014
RMSD bond angle	1.504
Data reduction software	XDS
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.690	36.690	2.320
High resolution limit [Å]	2.250	9.000	2.250
Rmerge	0.066	0.032	0.422
Rpim	0.021	0.011	0.132
Total number of observations	139352	2044	12890
Number of reflections	12703
<I/σ(I)>	22.5	39.5	6.5
Completeness [%]	100.0	98.4	100
Redundancy	11	8.7	11.1
CC(1/2)	0.999	0.999	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Lithium Sulphate, 20% PEG 3350