5DXT

p110alpha with GDC-0326

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99988
Spacegroup name	P 21 21 21
Unit cell lengths	58.507, 133.672, 141.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.120 - 2.250
R-factor	0.22812
Rwork	0.227
R-free	0.26196
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.051
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.120	2.330
High resolution limit [Å]	2.250	2.250
Rmerge		0.356
Number of reflections	51953
<I/σ(I)>	12.8	2.9
Completeness [%]	96.8	97.6
Redundancy	2.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 20000