5DXJ

Crystal structure of CARM1 and sinefungin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97914
Spacegroup name	P 21 21 2
Unit cell lengths	74.925, 97.881, 207.382
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.950
R-factor	0.1994
Rwork	0.198
R-free	0.23050
RMSD bond length	0.010
RMSD bond angle	1.529
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.020
High resolution limit [Å]	1.950	4.200	1.950
Rmerge	0.084	0.040	0.479
Total number of observations	518550
Number of reflections	110736
<I/σ(I)>	9.7
Completeness [%]	98.6	93.1	99.3
Redundancy	2.5	2.4	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG 3350