5DW7
Crystal structure of the unliganded geosmin synthase N-terminal domain from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.780, 76.780, 130.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.337 - 3.202 |
| R-factor | 0.2428 |
| Rwork | 0.240 |
| R-free | 0.28820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ps1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.670 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.340 | 3.316 |
| High resolution limit [Å] | 3.202 | 3.202 |
| Rmerge | 0.201 | 0.326 |
| Number of reflections | 6127 | |
| <I/σ(I)> | 6.99 | 4 |
| Completeness [%] | 89.1 | 78.52 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 277 | 0.1 M Tris, pH 8.2, 0.5 M magnesium chloride, 28% PEG400 |
