5DVM
Fc Design 20.8.37 B chain homodimer E357D/S364R/Y407A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-06-02 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.197, 107.197, 56.814 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.950 |
| R-factor | 0.2261 |
| Rwork | 0.223 |
| R-free | 0.28800 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.952 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 92.835 | 3.110 |
| High resolution limit [Å] | 2.950 | 9.330 | 2.950 |
| Rmerge | 0.083 | 0.796 | |
| Rmeas | 0.162 | ||
| Rpim | 0.062 | 0.037 | 0.631 |
| Total number of observations | 45883 | 1635 | 3235 |
| Number of reflections | 8056 | ||
| <I/σ(I)> | 7.9 | 16.5 | 1.1 |
| Completeness [%] | 98.7 | 99.1 | 92.3 |
| Redundancy | 5.7 | 5.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 100mM Sodium Cacodylate pH 6.5 + 20% PEG 8K + 200mM Magnesium Acetate tetrahydrate |
