5DUS
Crystal structure of MERS-CoV macro domain in complex with ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00545 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.798, 120.807, 67.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.530 - 1.432 |
| R-factor | 0.1295 |
| Rwork | 0.127 |
| R-free | 0.16190 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.213 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.530 |
| High resolution limit [Å] | 1.430 |
| Number of reflections | 31889 |
| <I/σ(I)> | 100.66 |
| Completeness [%] | 99.8 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | phosphate-citrate, ammonium sulfate, nicotinamide adenine dinucleotide |
